Leuko 24+ Spin Medium
Leuko 24+ Spin density gradient medium, sterile, ready-to-use solution for the in vitro isolation of all leukocytes from more than 12h old sample material (whole blood or buffy coat).
Details, Leuko 24+ Spin Medium
Leuko 24+ Spin density gradient medium is a sterile, ready-to-use solution for the isolation of human leukocytes from more than 12 h old sample material (whole blood or buffy coat) with high yield. Mononuclear and polymorphonuclear cells can be enriched with Leuko 24+ Spin Density Medium by simple density gradient centrifugation. When Leuko 24+ is combined with PBMC 24+ Spin Density Medium, the leukocyte population can be separated directly into PBMC and multinucleated cells.
Specification
Target cells
all leukocytes
Separation density cut-off
1,088 g/ml
Cell separation method
negative
Sample material
Human whole blood, Buffy Coat
Compatibility with
pluriMate, SepMate, LeucoSep
Can be used with
pluriSpin, MACS, EasySep, RosetteSep, MojoSort
Features
Leuko 24+ Spin Medium enables the enrichment of cells from sample material that is already older than 12 hours.
Alternative products, such as GranuloSep, Pancoll or Histopaque - 1119, for the enrichment of leukocytes can be substituted with Leuko Spin.
The enriched cells can be used for downstream applications such as MACS, SepMate, RosetteSep, MojoSort .
The pluriSpin separation media can be combined with each other and can be used, for example, as a double gradient for the simultaneous enrichment of two cell populations.
Separation Scheme
First, the sample material is carefully layered onto the Leuko Spin density gradient medium. Mixing of the two phases must be avoided. After density gradient centrifugation, the upper layer (plasma and dilution buffer) is aspirated. The two layers of leukocytes are then transferred to a new tube and the cells are washed.