pluriSpin Human Monocyte Enrichment
Isolation of human monocytes from whole blood, bone marrow, umbilical cord blood and buffy coat using negative selection.
Details pluriSpin human Monocyte enrichment, untouched cell separation
pluriSpin human Monocyte enrichment, for negative selection of cells, has been developed to isolate a clean, vital and untouched monocyte cell population from whole blood, buffy coat, cord blood, bone marrow or similar sample materials. The product labels the B-cells, T-cells, NK-cells and granulocytes, the monocyte fraction remains untouched.
Subsequent PBMC spin density gradient centrifugation is used to remove all labeled cells from the sample together with the red blood cells (RBCs) and granulocytes. The untouched and unlabeled monocyte fraction remains enriched in the interphase between the plasma and the density medium. We recommend PBMC Spin Medium as the density medium for DGM centrifugation. Alternatively, Ficoll, Lymphoprep or other comparable density media can be used without changing the protocol.
Specification
Target Cells
Monocytes
Cell separation method
negative
Compatible with
PBMC 24+ Spin Medium, Lymphoprep, Ficoll, Pancoll
Species
Human
Sample material
Whole blood, PBMC, PMNC, bone marrow, cell cultures
Cell separation platform
non-magnetic
Can be combined with
pluriMate or SepMate tubes
Features
Unwanted cells are labeled with specific antibodies and then removed, while the desired cells remain unlabeled in the suspension and retain their natural properties.
The isolated cells remain functional and can be used directly for subsequent experiments such as flow cytometry (FACS), cell culture or molecular biological analyses.
Unlike many other cell separation methods, pluriSpin does not require magnetic beads or columns, which simplifies the process and preserves cell integrity.
Purification by density centrifugation can be carried out using PBMC Spin Medium or comparable density media from other manufacturers, such as Lymphopreo, Ficoll or Pancoll.